METHODS IN ASSESSMENT OF EFFICACY OF INACTIVATED POLIOVIRUS VACCINES USING TRANSGENIC MICE SUSCEPTIBLE TO POLIOVIRUS

Abstract
This review concerns methodological approaches to assessment of protective properties of new inactivated poliovirus vaccines (IPV) using transgenic mice susceptible to poliovirus. Vaccines include a new generation of IPV that is made from attenuated Sabin strains (sIPV), as well as combination products that include other protective antigens. Authors describe their own results and data published by others in order to present comprehensive picture of this assessment. The transgenic mouse test is based on immunization-challenge (functional part of the test) and includes serological assessment. Comparison of conventional IPV (cIPV) and sIPV of type 1 showed that type 1 sIPV possesses excellent immunogenicity and protectivity in Tg-mice. It was found that sIPV of type 2 induced lower levels of neutralizing antibodies and was less protective than type 2 cIPV against challenge with wild-type poliovirus. Authors conclude that transgenic mouse test for evaluation of immunogenicity and protectivity of new IPVs is an effective surrogate method in situation when clinical efficacy trials in people are impossible.

Key words
poliovirus vaccine, animal model, protectivity, serological tests

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