Resumen
Dados epidemiológicos, sorológicos e moleculares permitem um melhor conhecimento da circulação do HAV na população e podem contribuir para o controle desta doença. Nesta revisão, o desenvolvimento de métodos de biologia molecular para o estudo desta virose, tanto para fins de diagnóstico como para estudos de epidemiologia molecular são discutidos. Quatro métodos diferentes de extração do RNA do HAV foram comparados. A partir da padronização do RT-PCR, foi realizada a detecção do RNA do HAV em amostras de soro de casos de hepatite sem o marcador anti-HAV IgM para definir o diagnóstico precoce de hepatite A na fase pré-soroconversão e esclarecer de casos de hepatite sem etiologia conhecida. O seqüenciamento nucleotídico de isolados de HAV mostrou que a maioria das cepas brasileiras é classificada no genotipo I, com co-circulação dos subgenótipos IA e IB, também mostramos um caso de infecção mista descrito pela primeira vez na literatura. Para facilitar a genotipagem de isolados de HAV, padronizamos a técnica de "heteroduplex mobility assay" o que simplificou nosso trabalho com a mesma eficiência. A detecção do RNA do HAV como ferramenta diagnóstica possibilitou também a identificação de casos de co-infecção de hepatite A em pacientes portadores de hepatite C.
Clasificación en siicsalud
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Especialidades
Principal: Diagnóstico por Laboratorio
Relacionadas: Bioquímica, Epidemiología, Infectología, Medicina Interna, Salud Pública
Enviar correspondencia a:
Vanessa S. de Paula, Fundação Oswaldo Cruz, Río de Janeiro, Brasil
Patrocinio y reconocimiento
Os procedimentos foram feitos de acordo aos padrões éticos de experimentação em seres humanos da Declaração de Helsinque e aprovados pelo comitê de ética da Fundação Oswaldo Cruz.
Agradecimentos: Ao CNPq e a FAPERJ pelo financiamento dos projetos, a Dra. Ana Maria Coimbra Gaspar, ao Dr. Christian Niel e a Dra. Livia Melo Villar pela orientação e colaboração nos projetos de pesquisa.
Artículo completo
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Extensión:
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Key words
hepatitis A virus, detection, PCR, HMA and genotype
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