IDENTIFICAN PROTEINAS ESPERMATICAS INVOLUCRADAS EN LA FECUNDACION HUMANA





IDENTIFICAN PROTEINAS ESPERMATICAS INVOLUCRADAS EN LA FECUNDACION HUMANA

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Los anticuerpos presentes en fluidos foliculares humanos con capacidad de alterar la funcionalidad espermática constituyen una herramienta para la identificación de proteínas involucradas en la interacción de los gametos.
briggiler9.jpg Autor:
Clara Isabel Marín Briggiler
Columnista Experto de SIIC

Institución:
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)


Artículos publicados por Clara Isabel Marín Briggiler
Coautores
María Fernanda González Echeverría* Jorge Blaquier** Jorge Guillermo Tezón*** Mónica Hebe Vázquez-Levin**** 
Lic. en Ciencias Biológicas, Laboratorio de Reproducción Asistida, Centro Médico Fertilab, Buenos Aires, Argentina*
Dr. en Ciencias Médicas, Centro Médico Fertilab, Buenos Aires, Argentina**
Dr. en Ciencias Químicas, Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina***
Dra. en Ciencias Químicas, Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina****
Recepción del artículo
11 de Abril, 2006
Aprobación
2 de Mayo, 2006
Primera edición
28 de Julio, 2006
Segunda edición, ampliada y corregida
7 de Junio, 2021

Resumen
La presencia de anticuerpos antiespermáticos en diferentes fluidos biológicos puede afectar la interacción entre el espermatozoide y el ovocito y causar infertilidad. En trabajos previos de nuestro grupo se demostró que los fluidos foliculares de pacientes participantes de un programa de fecundación in vitro contienen inmunoglobulinas (IgGFF) con capacidad de alterar la función espermática, esto es, tanto inhibir la unión de los espermatozoides a la matriz extracelular del ovocito como inducir la liberación de las enzimas acrosomales (Marín Briggiler et al, Am J Reprod Immunol, 2003). El objetivo del presente estudio fue utilizar dichos anticuerpos para identificar proteínas espermáticas involucradas en la interacción de los gametos humanos. Se llevaron a cabo ensayos de Western immunoblotting en los que proteínas extraídas de espermatozoides humanos incubados en condiciones capacitantes fueron separadas por electroforesis en geles de poliacrilamida de una dimensión, transferidas a membranas de nitrocelulosa y expuestas a fluidos foliculares individuales y anticuerpos anti-IgG humana. Tres de las IgGFF capaces de alterar los eventos relacionados con la interacción de los gametos reconocieron proteínas espermáticas de 78 ± 1, 58 ± 2 y 15 ± 1 kDa. La caracterización bioquímica y molecular de estos polipéptidos permitirá confirmar su participación en la interacción espermatozoide-ovocito. Estos estudios contribuirían a dilucidar las bases moleculares del proceso de fecundación y conducirían a proponer métodos alternativos para el diagnóstico y tratamiento de la infertilidad, así como nuevas estrategias para la regulación de la natalidad.

Palabras clave
proteínas espermáticas, anticuerpos antiespermáticos, interacción de gametas, reproducción humana, fluido folicular


Artículo completo

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Extensión:  +/-6.48 páginas impresas en papel A4
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Abstract
Presence of antisperm antibodies in biological fluids can alter sperm-egg interaction and cause infertility. Previous studies from our group have demonstrated that follicular fluids obtained from patients participating in an in vitro fertilization program contain immunoglobulins (FFIgG) with the ability to affect sperm function, i.e. to inhibit sperm binding to the egg extracellular matrix and/or to induce the release of acrosomal enzymes (Marín Briggiler et al, Am J Reprod Immunol, 2003). The objective of the present study was to use these antibodies to identify sperm proteins involved in human gamete interaction. Western immunoblot assays were carried out in which proteins extracted from human sperm previously incubated under capacitating conditions were separated by one dimensional polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes and exposed to individual follicular fluids and anti human IgG antibodies. Three of the FFIgG with the capacity of altering gamete interaction-related events recognized sperm proteins of 78 ± 1, 58 ± 2 and 15 ± 1 kDa. Biochemical and molecular characterization of these polypeptides will confirm their participation in sperm-egg interaction. These studies would contribute to elucidate the molecular basis of the fertilization process, and would lead to propose alternative methods for infertility diagnosis and treatment, as well as new strategies for fertility regulation.

Key words
sperm proteins, antisperm antibodies, gamete interaction, human reproduction, follicular fluid


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Inmunología
Relacionadas: Bioquímica, Diagnóstico por Laboratorio, Urología



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Enviar correspondencia a:
Clara I. Marín Briggiler, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1428ADN, Vuelta de Obligado 2490, Buenos Aires, Argentina
Patrocinio y reconocimiento:
El estudio fue subsidiado con fondos de la Organización Mundial de la Salud (LID Grant # 97175), el Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y la Agencia Nacional de Promoción de la Ciencia y Tecnología (PICT97, 00207) (Argentina).
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