Juan Antonio Rosado *

Autor invitado por SIIC

El trabajo contribuye a esclarecer el papel de las balsas lipídicas en la entrada de calcio

LAS BALSAS LIPÍDICAS REGULAN LA ACTIVACIÓN PERO NO EL MANTENIMIENTO DE LA ENTRADA CAPACITATIVA DE CALCIO

Las balsas lipídicas son necesarias para la activación de la entrada capacitativa de calcio mediada por la formación del complejo STIM1-Orai1-TRPC1-TRPC6, pero una vez se ha formado el complejo de señalización, la desorganización de las balsas lipídicas no afecta a la entrada de calcio.

*Juan Antonio Rosado
describe para SIIC los aspectos relevantes de su trabajo
LIPID RAFTS MODULATE THE ACTIVATION BUT NOT THE MAINTENANCE OF STORE-OPERATED CA2+ ENTRY
Institución principal de la investigación
*Universidad de Extremadura, Caceres, España
Imprimir nota

Referencias bibliográficas
Cheng, K.T., Ong, H.L., Liu, X., Ambudkar, I.S., 2011. Contribution of TRPC1 and Orai1 to Ca(2+) entry activated by store depletion. Advances in experimental medicine and biology 704, 435-449. Desai, P.N., Zhang, X., Wu, S., Janoshazi, A., Bolimuntha, S., Putney, J.W., Trebak, M., 2015. Multiple types of calcium channels arising from alternative translation initiation of the Orai1 message. Science signaling 8, ra74. Feske, S., Gwack, Y., Prakriya, M., Srikanth, S., Puppel, S.H., Tanasa, B., Hogan, P.G., Lewis, R.S., Daly, M., Rao, A., 2006. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 441, 179-185. Galan, C., Woodard, G.E., Dionisio, N., Salido, G.M., Rosado, J.A., 2010. Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry. Biochimica et biophysica acta 1803, 1083-1093. Jardin, I., Gomez, L.J., Salido, G.M., Rosado, J.A., 2009. Dynamic interaction of hTRPC6 with the Orai1/STIM1 complex or hTRPC3 mediates its role in capacitative or non-capacitative Ca2+ entry pathways. Biochem J 420, 267-276. Jardin, I., Salido, G.M., Rosado, J.A., 2008. Role of lipid rafts in the interaction between hTRPC1, Orai1 and STIM1. Channels (Austin) 2, 401-403. Noble, D., Borysova, L., Wray, S., Burdyga, T., 2014. Store-operated Ca(2)(+) entry and depolarization explain the anomalous behaviour of myometrial SR: effects of SERCA inhibition on electrical activity, Ca(2)(+) and force. Cell calcium 56, 188-194. Pani, B., Ong, H.L., Liu, X., Rauser, K., Ambudkar, I.S., Singh, B.B., 2008. Lipid rafts determine clustering of STIM1 in endoplasmic reticulum-plasma membrane junctions and regulation of store-operated Ca2+ entry (SOCE). J Biol Chem 283, 17333-17340. Putney, J.W., Jr., 1986. A model for receptor-regulated calcium entry. Cell Calcium 7, 1-12. Roos, J., DiGregorio, P.J., Yeromin, A.V., Ohlsen, K., Lioudyno, M., Zhang, S., Safrina, O., Kozak, J.A., Wagner, S.L., Cahalan, M.D., Velicelebi, G., Stauderman, K.A., 2005. STIM1, an essential and conserved component of store-operated Ca2+ channel function. J Cell Biol 169, 435-445. Rosado, J.A., Brownlow, S.L., Sage, S.O., 2002. Endogenously expressed Trp1 is involved in store-mediated Ca2+ entry by conformational coupling in human platelets. J Biol Chem 277, 42157-42163. Vaca, L., 2010. SOCIC: the store-operated calcium influx complex. Cell Calcium 47, 199-209. Zhang, S.L., Yu, Y., Roos, J., Kozak, J.A., Deerinck, T.J., Ellisman, M.H., Stauderman, K.A., Cahalan, M.D., 2005. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 437, 902-905.

Otros artículos de Juan Antonio Rosado

1: Smani T, Shapovalov G, Skryma R, Prevarskaya N, Rosado JA. Functional and
physiopathological implications of TRP channels. Biochim Biophys Acta. 2015
Aug;1853(8):1772-82. doi: 10.1016/j.bbamcr.2015.04.016.

2: Dionisio N, Smani T, Woodard GE, Castellano A, Salido GM, Rosado JA. Homer
proteins mediate the interaction between STIM1 and Cav1.2 channels. Biochim
Biophys Acta. 2015 May;1853(5):1145-53. doi: 10.1016/j.bbamcr.2015.02.014.

3: Albarrán L, Dionisio N, Lopez E, Salido GM, Redondo PC, Rosado JA. STIM1
regulates TRPC6 heteromultimerization and subcellular location. Biochem J. 2014
Nov 1;463(3):373-81. doi: 10.1042/BJ20140523.

4: López J, Dionisio N, Berna-Erro A, Galán C, Salido GM, Rosado JA. Two-pore
channel 2 (TPC2) modulates store-operated Ca(2+) entry. Biochim Biophys Acta.
2012 Oct;1823(10):1976-83. doi: 10.1016/j.bbamcr.2012.08.002.

5: Zbidi H, Jardin I, Woodard GE, Lopez JJ, Berna-Erro A, Salido GM, Rosado JA.
STIM1 and STIM2 are located in the acidic Ca2+ stores and associates with Orai1
upon depletion of the acidic stores in human platelets. J Biol Chem. 2011 Apr
8;286(14):12257-70. doi: 10.1074/jbc.M110.190694.

6: Rosado JA, Pariente JA, Salido GM, Redondo PC. SERCA2b activity is regulated
by cyclophilins in human platelets. Arterioscler Thromb Vasc Biol. 2010
Mar;30(3):419-25. doi: 10.1161/ATVBAHA.109.194530.

7: Woodard GE, López JJ, Jardín I, Salido GM, Rosado JA. TRPC3 regulates
agonist-stimulated Ca2+ mobilization by mediating the interaction between type I
inositol 1,4,5-trisphosphate receptor, RACK1, and Orai1. J Biol Chem. 2010 Mar
12;285(11):8045-53. doi: 10.1074/jbc.M109.033605.

8: Jardin I, Lopez JJ, Salido GM, Rosado JA. Orai1 mediates the interaction
between STIM1 and hTRPC1 and regulates the mode of activation of hTRPC1-forming
Ca2+ channels. J Biol Chem. 2008 Sep 12;283(37):25296-304. doi:
10.1074/jbc.M802904200.

9: López JJ, Salido GM, Pariente JA, Rosado JA. Interaction of STIM1 with
endogenously expressed human canonical TRP1 upon depletion of intracellular Ca2+
stores. J Biol Chem. 2006 Sep 22;281(38):28254-64.

10: Rosado JA, Meijer EM, Hamulyak K, Novakova I, Heemskerk JW, Sage SO.
Fibrinogen binding to the integrin alpha(IIb)beta(3) modulates store-mediated
calcium entry in human platelets. Blood. 2001 May 1;97(9):2648-56.

Para comunicarse con Juan Antonio Rosado mencionar a SIIC como referencia:
jarosado@unex.es

---

Acerca del trabajo completo

LAS BALSAS LIPÍDICAS REGULAN LA ACTIVACIÓN PERO NO EL MANTENIMIENTO DE LA ENTRADA CAPACITATIVA DE CALCIO

Título original en castellano
LAS BALSAS LIPIDICAS REGULAN LA ACTIVACION PERO NO EL MANTENIMIENTO DE LA ENTRADA CAPACITATIVA DE CALCIO

Autor
Juan Antonio Rosado¹, Carmen Galan², Geoffrey Woodard³, Natalia Dionisio⁴, Gines Salido⁵
1 Profesor Titular de Universida, Universidad de Extremadura, Caceres, España, Profesor
2 Tecnico de Laboratorio, Universidad de Extremadura
3 Investigador, Niddk, Nih
4 Profesora
5 Catedrático de Universidad, Universidad de Extremadura

Acceso a la fuente original
BBA Molecular Cell Research
http://www.elsevier.com/wps/find/journaldescription.cws_home/506069/description