Aline Helena da Silva Cruz *

Autora invitada por SIIC

O trabalho contribui para o entendimento da regulação da enzima isocitrato liase

ISOCITRATO LIASE DE PARACOCCIDIOIDES BRASILIENSIS É REGULADA POR FOSFORILAÇÃO

Os resultados sugerem um novo mecanismo de regulação do ciclo do glioxalato em fungos, o qual é independente da regulação transcricional, mas controlado por modificação pós-traducional.

*Aline Helena da Silva Cruz
describe para SIIC los aspectos relevantes de su trabajo
PHOSPHORYLATION IS THE MAJOR MECHANISM REGULATING ISOCITRATE IYASE ACTIVITY IN PARACOCCIDIOIDES BRASILIENSIS YEAST CELLS
Institución principal de la investigación
*Mestre, Goiânia, Brasil
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Referencias bibliográficas
Ebel F, Schwienbacher M, Beyer J, Heesemann J, Brakhage AA & Brock M (2006) Analysis of the regulation, expression, and localization of the isocitrate lyase from Aspergillus fumigatus, a potential target for antifungal drug development. Fungal Genet Biol 43, 476–489.

Fernandez E, Fernandez M, Moreno F & Rodicio R (1993) Transcriptional regulation of the isocitrate lyase encoding gene in Saccharomyces cerevisiae. FEBS Lett 333, 238–242.

Wayne LG & Lin KY (1982) Glyoxylate metabolism and adaptation of Mycobacterium tuberculosis to survival under anaerobic conditions. Infect Immun 37, 1042–1049.

Rude TH, Toffaletti DL, Cox GM & Perfect JR (2002) Relationship of the glyoxylate pathway to the pathogenesis of Cryptococcus neoformans. Infect Immun 70, 5684–5694.

Bentrup KHZ, Miczak A, Swenson DL & Russel DG (1999) Characterization of activity and expression of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis. 181, 7161-7167.

McKinney JD, Honer zu Bentrup K, Munñoz-Elías EJ, Miczak A, Chen B, Chan WT, Swenson D, Sacchettini JC, Jacobs WR Jr & Russell DG (2000) Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase. Nature 406, 735–738.

Muñoz-Elías EJ & McKinney JD (2005) Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence. Nat Med 11, 638–644.

Lorenz MC & Fink GR (2001) The glyoxylate cycle is required for fungal virulence. Nature 412, 83–86.

Wang ZY, Thornton CR, Kershaw MJ, Debao L & Talbot NJ (2003) The glyoxylate cycle is required for temporal regulation of virulence by the plant pathogenic fungus Magnaporthe grisea. Mol Microbiol 47, 1601–1612.

Idnurm A & Howlett BJ (2002) Isocitrate lyase is essential for pathogenicity of the fungus Leptosphaeria maculans to canola (Brassica napus). Eukaryot Cell 1, 719–724.

Brummer E, Castaneda E & Restrepo A (1993) Paracoccidioidomycosis: an update. Clin Microbiol Rev 6, 89-117.

Felipe MS, Andrade RV, Arraes FB, Nicola AM, Maranhão AQ, Torres FA, Silva-Pereira I, Pocas-Fonseca MJ, Campos EG, Moraes LM et al. (2005) Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells. J Biol Chem 280, 24706–24714.

Nunes LR, Costa de Oliveira R, Leite DB, da Silva VS, dos Reis Marques E, da Silva Ferreira ME, Ribeiro DC, de Souza Bernardes LA, Goldman MH, Puccia R et al. (2005) Transcriptome analysis of Paracoccidioides brasiliensis cells undergoing mycelium-to-yeast transition. Eukaryot Cell 4, 2115–2128.

Bastos KP, Bailão AM, Borges CL, Faria FP, Felipe MS, Silva MG, Martins WS, Fiuza RB, Pereira M & Soares CM (2007) The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process. BMC Microbiol 7, 29.

Costa M, Borges CL, Bailão AM, Meirelles GV, Mendonca YA, Dantas SF, de Faria FP, Felipe MS, Molinari-Madlum EE, Mendes-Giannini MJ et al. (2007) Transcriptome profiling of Paracoccidioides brasiliensis yeast-phase cells recovered from infected mice brings new insights into fungal response upon host interaction. Microbiology 153, 4194–4207.

Otros artículos de Aline Helena da Silva Cruz

PEREIRA, M.; Song, Z.; SANTOS-SILVA, L.K.; RICHARDS, M.H.; NGUYEN, T.T.; LIU, J.; SOARES, C.M.A.; CRUZ, A.H.S.; GANAPATHY, K.; NES, W.D. (2010) Cloning, Mechanistic and Functional Analysis of a Fungal Sterol 24C- Methyltransferase Implicated in Brassicasterol Biosynthesis. BBA. Molecular and Cell Biology of Lipids 1801, 1163-1174.

TOMAZETT, P.K.; CASTRO, N,S.; LENZI, H.L., SOARES, C.M.A.; PEREIRA, M. (2011) Response of Paracoccidioides brasiliensis Pb01 to stressor agents and cell wall osmoregulators. Fungal Biology 115, 62-69.

TOMAZETT, P.K.; FELIX, C.R.; LENZI, H.L., FARIA, F.P.; SOARES, C.M.A.; PEREIRA, M. (2010) 1,3-β-D-glucan synthase of Paracoccidioides brasiliensis: recombinant protein, expression and cytolocalization in yeast and mycelium phases. Fungal Biology 114, 809-16.

ZAMBUZZI-CARVALHO, P.F.; CRUZ, A.H.S.; SILVA, L.K.S.; GOES, A.; SOARES, C.M.A.; PEREIRA, M. (2009) The malate synthase of Paracoccidioides brasiliensis Pb01 is required in the glyoxylate cycle and in the allantoin degradation pathway. Medical Mycology 31, 1-11.

NETO, B.R.S., SILVA, J.F., MENDES-GIANNINI, M.J.S., LENZI, H.L., SOARES, C.M.A., PEREIRA, M. (2009) The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin. BMC Microbiol. 9:272.

SANTOS, G.D.; FERRI, P.H. ; SANTOS, S.C.; BAO, S.N.; SOARES, C.M.A.; PEREIRA, M. (2007) Oenothein B inhibits the expression of PbFKS1 transcript and induces morphological changes in Paracoccidioides brasiliensis. Medical Mycology 45, 609-618.

OKAMOTO, H.T.S.; SOARES, C.M.A.; PEREIRA, M. (2006) Comparative analyses of the structure of the 1,3-Beta-glucan synthase in Paracoccidioides brasiliensis isolates. Genetics and Molecular Research 5, 407-418.

BONFIM, S.M.R.C.; CRUZ, A.H.S.; JESUINO, R.S.A.; ULHOA, C.J.; MOLINARIMADLUM, E. e W.I.; SOARES, C.M.A.; PEREIRA, M. (2006) Chitinase from Paracoccidioides brasiliensis: Molecular cloning, structural, phylogenetic, expression and activity analysis. FEMS Immunology and Medical Microbiology 46, 269-283.

Para comunicarse con Aline Helena da Silva Cruz mencionar a SIIC como referencia:
maristelaufg@gmail.com

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Acerca del trabajo completo

ISOCITRATO LIASE DE PARACOCCIDIOIDES BRASILIENSIS É REGULADA POR FOSFORILAÇÃO

Título original en castellano
FOSFORILAÇÃO E O PRINCIPAL MECANISMO DE REGULAÇÃO DA ATIVIDADE DA ISOCITRATO LIASE EM CELULAS LEVEDURIFORMES DE PARACOCCIDIOIDES BRASILIENSIS.

Autor
Aline Helena da Silva Cruz¹, Matthias Brock², Patrícia Fernanda Zambuzzi-Carvalho³, Ludier Kesser Santos-Silva⁴, Rogério Fraga Troian⁵, Alfredo Miranda Góes⁶, Célia Maria De Almeida Soares⁷, Maristela Pereira⁸
1 Doutoranda, Mestre, Goiânia, Brasil, Aluna
2 Pesquisador, Leibniz Institute For Natural Product Research And Infection Biology (hki), Pesquisador
3 Pós-doutoranda, Universidade Federal de Goiás, Pós-doutoranda
4 Doutoranda, Universidade Federal de Goiás, Doutoranda
5 Doutorando, Universidade Federal de Goiás, Doutorando
6 Professor, Universidade Federal de Minas Gerais, Professor
7 Professor, Universidade Federal de Goiás, Professor
8 Professor, Universidade Federal de Goiás, Professor

Acceso a la fuente original
FEBS Journal
http://www.febsjournal.org