SUSCEPTIBILIDADE A ANTIFUNGICOS E PRODUÇAO DE ENZIMAS POR LEVEDURAS DO (LEVADURAS DEL) GENERO CANDIDA ISOLADAS (AISLADAS) DE PACIENTES COM HIV/AIDS

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A candidíase é a (constituye la) infecção fúngica mais frequente entre os pacientes portadores do vírus da imunodeficiência humana.
pupulin9.jpg Autor:
Aurea Regina Pupulin
Columnista Experta de SIIC

Institución:
Universidade Estadual de Maringá (UEM)


Artículos publicados por Aurea Regina Pupulin
Coautores
Paula Galdino Carvalho* Celso Vataru Nakamura** 
Mestre, Universidade Estadual de Maringá (UEM), Maringá, Brasil*
PhD, Universidade Estadual de Maringá (UEM), Maringá, Brasil**
Recepción del artículo
27 de Septiembre, 2011
Aprobación
5 de Octubre, 2012
Primera edición
18 de Octubre, 2012
Segunda edición, ampliada y corregida
7 de Junio, 2021

Resumen
A candidíase é a (constituye la) infecção fúngica mais frequente entre os pacientes portadores do vírus da imunodeficiência humana. Este estudo objetivou o isolamento (el aislamiento), identificação, avaliação do (evaluación del) perfil de susceptibilidade e fatores de virulência de leveduras do gênero Candida provenientes de pacientes HIV/AIDS. As amostras foram coletadas (Las muestras se recolectaron) de 100 pacientes em diferentes sítios anatômicos (boca, trato gastrointestinal e vaginal), cultivadas em meio (en medio) ágar Sabouraud, e identificadas através da cultura em meio (a través de cultivo en medio) diferencial CHROMágar Candida e pela técnica da reação em cadeia da (y por la técnica de reacción en cadena de la) polimerase (PCR). O teste de (La prueba de) susceptibilidade in vitro aos (a los) antifúngicos fluconazol, anfotericia B e nistatina foram determinados através da técnica de microdiluição em caldo (en caldo). A infectividade foi avaliada através da (se validó por medio de la) produção de fosfolipases e hemolisinas. Do total (De un total) de 45 leveduras isoladas, houve maior (hubo mayor) prevalência de C. albicans, seguida por C. glabrata, C.parapisilosis, C.tropicalis e C. krusei. Foram submetidas ao (Se sometieron a la) teste de suscptibilidade 43 (95%) das amostras, onde a (en la que la) concentração inibitória mínima foi determinada. Todos os isolados foram sensíveis a (Todos los aislamientos eran sensibles a) anfotericina B e a nistatina, enquanto 60.6% foram sensíveis ao fluconazol, 6.9% sensíveis dose-dependente e 32.5% apresentaram resistência ao (presentaron resistencia al) fluconazol. As espécies C. glabrata e C. tropicalis apresentaram CIMs maior para o fluconazol do que os encontrados para C. albicans. Apresentou produção de fosfolipase 62% das amostras e atividade hemolítica 87%.

Palabras clave
Candida sp, HIV/AIDS, antifúngicos


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Abstract
Thrush or Candida albicans is one of the most common fungal infections among patients with human immunodeficiency virus. The isolation, identification and evaluation of susceptibility and virulence factors of Candida yeasts from HIV/AIDS patients are provided. Samples, collected from different anatomical sites (mouth, gastrointestinal tract and vagina) of 100 patients, were grown in Sabouraud agar and identified by culture in differential medium CHROMagar Candida and by polymerase chain reaction (PCR) technique. The in vitro susceptibility test for antifungal fluconazole, amphotericin B and nystatin were determined by broth micro-dilution technique. Infectivity was assessed through the production of phospholipases and hemolysins. Candida albicans was the most prevalent among the 45 isolated yeasts, followed by C. glabrata, C. parapisilosis, C. tropicalis and C. krusei. Forty-three (95%) samples were tested for susceptibility in which minimum inhibitory concentration was determined. All isolates were susceptible to amphotericin B and nystatin, whereas 60.6% were susceptible to fluconazole; 6.9% were susceptible-dose dependent and 32.5% were resistant to fluconazole. The species C. glabrata and C. tropicalis showed higher CIMs for fluconazole than those found for C. albicans. Furthermore, 62% of samples produced phospholipase and 87% produced hemolytic activity.

Key words
Candida sp, HIV/AIDS, antifungal


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Diagnóstico por Laboratorio, Infectología
Relacionadas: Bioquímica, Epidemiología, Medicina Interna



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Enviar correspondencia a:
Aurea Regina Pupulin, Universidade Estadual de Maringá (UEM), Av Colombo 5790 bloco I-90 DBS/parasitologia, Maringá, Brasil
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