LOS VIRUS HERPES HUMANOS 4 (VIRUS DE EPSTEIN BARR/VEB) Y 6 (HHV-6) EN LAS ENFERMEDADES DE HODGKIN Y DE KIKUCHI, Y SU RELACION CON PROLIFERACION Y APOPTOSIS





LOS VIRUS HERPES HUMANOS 4 (VIRUS DE EPSTEIN BARR/VEB) Y 6 (HHV-6) EN LAS ENFERMEDADES DE HODGKIN Y DE KIKUCHI, Y SU RELACION CON PROLIFERACION Y APOPTOSIS

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La Enfermedad de Hodgkin está frecuentemente infectada tanto por Virus Epstein Barr como por Herpes Virus 6 mientras que la Enfermedad de Kikuchi tiende a estar infectada únicamente por el Herpes Virus 6.
rojo9.jpg Autor:
Julieta Rojo
Columnista Experto de SIIC
Artículos publicados por Julieta Rojo
Coautores
Mónica Romero (MD)*  Humberto Cruz-Ortíz (MD)*  Marie Luise Huetter (MD)**  Birgit Gathof (MD)***  GRF Krueger (MD)**** 
Hospital General de México, O.D. Depto. de Salud Pública y Unidad de Patología , Facultad de Medicina de la Universidad Nacional Autónoma de México (UNAM)*
Laboratorio de Inmunopatología, Clínica Universidad de Colonia, Alemania**
Medicina Transfusional. Clínica de la Universidad de Colonia, Alemania.***
Instituto de Anatomía. Universidad de Colonia,Alemania.****
Recepción del artículo
17 de Noviembre, 2003
Aprobación
5 de Enero, 2004
Primera edición
10 de Febrero, 2004
Segunda edición, ampliada y corregida
7 de Junio, 2021

Resumen
Antecedentes. El virus de Epstein Barr (VEB) y el herpesvirus humano tipo 6 HHV-6 se encuentran frecuentemente asociados a la enfermedad de Hodgkin (EH) y en algunos casos a la de Kikuchi Fujimoto (EKF). Ambos tienen una relación aparente con procesos de proliferación o de apoptosis como lo representan la EH y EKF respectivamente. Objetivo. En el presente trabajo investigamos la frecuencia y localización de la expresión antigénica y del ADN de ambos virus en cortes de tejido de EH y EKF en relación a marcadores de proliferación celular y de apoptosis. Método. Se revisaron las biopsias de ganglios de 84 pacientes del Hospital General de México y de 26 de la Clínica de la Universidad de Colonia con EH y 14 con EKF; todas fueron investigadas inmunohistológicamente para expresión antigénica viral (VEB: LMP-1: HHV-6 gp110/60), Ki67/PCNA, marcador de proliferación (MIB1)/p53 y WAF1 para apoptosis. El ADN viral se demostró por hibridación in situ. La apoptosis se demostró con las técnicas de ISEL y TUNEL. Resultados. La EH está frecuentemente infectada tanto por VEB como por HHV-6 mientras que la EKF tiende a estar infectada únicamente por el HHV-6. El VEB está presente en la EH en las células de Hodgkin y de Reed-Sternberg y el HHV-6 en linfocitos e histiocitos de ambas enfermedades. El marcador de proliferación celular se encontró en ambas enfermedades . La apoptosis se demostró principalmente en la EKF y menos en la EH. Conclusión. Aun cuando el VEB y el HHV-6 pueden no ser abiertamente oncogénicos en la EH y la EKF, pueden influir en el curso de la enfermedad. La infección dual en la EH parece apoyar más el proceso de proliferación, con predominio del efecto del VEB. La infección por HHV-6 en la EKF parece favorecer el curso apoptósico. Estos efectos son -de acuerdo con la literatura- posiblemente mediados por citocinas.

Palabras clave
Enfermedad de Hodgkin, enfermedad de Kikuchi-Fujimoto, virus de Epstein Barr (VEB), virus herpes humano-6 (HHV6), apoptosis, proliferación


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Abstract
Background: Epstein Barr virus/EBV and HHV-6 are frequently found in Hodgkin's disease (HD) and -to a certain extent- in Kikuchi-Fujimoto's disease (KFD). Both viruses are apparently related to proliferative and/or apoptotic processes as represented by HD or KFD respectively. Objective: To correlate frequency and location of antigen and DNA expression of both viruses in HD or KFD tissue sections in relation to markers for cell proliferation and apoptosis. Design: Archival lymph node biopsies from 84 patients from the Hospital General de Mexico and 26 from the Clinic of the University of Cologne, Germany with HD and 14 with KFD were investigated immunhistologically for viral antigen expression (EBV LMP-1: HHV-6 gp110/60), Ki67/PCNA marker for proliferation (MIB1)/p53 and WAF1 for apoptosis. Viral ADN was shown by in situ hybridization. Apoptosis was determined by ISEL and TUNEL techniques. Results: HD is frequently infected by both EBV and HHV-6 while KFD tends to be infected only by HHV-6. EBV and HD is present in HD cells and Reed-Sternberg cells, HHV-6 preferentially in lymphocytes and in histiocytes in both HD and in KFD. Proliferation marker is found in both diseases. Apoptosis is demonstrated preferentially in KFD and to a lesser extent also in HD. Conclusion: Although EBV and HHV-6 may not be openly oncogenic in HD and KFD, they may well influence the course of the disease. Dual infection in HD appears to support proliferative processes,i.e. a predominance of EBV effects. Single infection with HHV-6 in KFD instead appears to favor an apoptotic course. These effects are- according to the literature-possibly cytokine-mediated.

Key words
Hodgkin disease, Kikuchi Fujimoto disease, Epstein Barr Virus (EVB), human Herpesvirus-6 (HHV-6), apoptosis, proliferation


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Oncología
Relacionadas: Anatomía Patológica, Hematología, Infectología, Medicina Interna



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Este trabajo fue realizado en parte gracias al apoyo del Servicio Alemán de Intercambio Académico (DAAD).
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