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BACTERIOTERAPIA CON LACTOBACILOS PROBIOTICOS PARA LA PREVENCION DE INFECCIONES UROGENITALES

BACTERIOTERAPIA CON LACTOBACILOS PROBIOTICOS PARA LA PREVENCION DE INFECCIONES UROGENITALES

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La administración intrauretral de lactobacilos no produjo efectos adversos ni modificaciones estructurales o ultraestructurales en un modelo murino de investigación.
nadermacias9.jpg Autor:
María Elena Nader-macias
Columnista Experto de SIIC

Institución:
Centro de Referencia para Lactobacilos (CERELA-CONICET)


Artículos publicados por María Elena Nader-macias
Recepción del artículo
2 de Junio, 2005
Aprobación
13 de Junio, 2005
Primera edición
6 de Febrero, 2006
Segunda edición, ampliada y corregida
7 de Junio, 2021

Resumen
Los lactobacilos son los microorganismos predominantes en el tracto gastrointestinal y urogenital del hombre y los animales homeotermos. También se emplean en la elaboración de diferentes alimentos, lo que los categoriza como GRAS (generally regarded as safe). Si bien están disponibles en productos vaginales en el mercado farmacéutico, hay escasas evidencias de su efectividad para la prevención de infecciones urogenitales. Nuestro grupo de trabajo ha aislado lactobacilos vaginales humanos, seleccionando aquellos con propiedades benéficas o probióticas. Se han estudiado las características tecnológicas para obtener el mayor número de microorganismos vivos (biomasa) y las condiciones para la producción óptima de sustancias antagónicas frente a patógenos urogenitales (ácidos orgánicos, peróxido de hidrógeno, bacteriocinas). Se ha demostrado en animales de experimentación (ratones BALB/c hembra endocriados) que la administración intrauretral de lactobacilos no produce efectos adversos ni modificaciones estructurales o ultraestructurales, previniendo eficazmente las infecciones urinarias. Es necesaria la complementación de estos resultados con estudios en pacientes para demostrar la efectividad de la administración intravaginal de los lactobacilos probióticos seleccionados en la recolonización de la microbiota urogenital, la prevención o la terapia de los diferentes síndromes y cuadros clínicos que afectan al tracto, su efecto sobre las embarazadas, etc., que permitan proponer la bacterioterapia con lactobacilos como alternativa válida a la terapia con otras drogas.

Palabras clave
Lactobacilos, prevención, infecciones urogenitales, probióticos


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Abstract
Lactobacilli are the predominant microorganisms in the gastrointestinal and urogenital tracts of human beings and homeotermic animals. They are also used for the elaboration of different fermented foods being categorized as GRAS (generally regarded as safe). Even though they are included into different vaginal products in the pharmaceutical market, there are small amount of evidences of their effectivity in the prevention of urogenital infections. Our research group have isolated human vaginal lactobacilli, selecting those with beneficial or probiotic properties. The technological characteristics to obtain the higher number of viable (biomass) microorganisms and the conditions for the optimal production of antagonistic substances against pathogenic microorganisms (organic acid, hydrogen peroxide, bacteriocins) were determined. In experimental animals (inbreed female BALB/c mice) we demonstrated that the intraurethral administration of lactobacilli does not produce adverse effects or structural or ultra structural modifications in the urogenital tract, preventing effectively the urinary tract infections. The complementation of these results with human assays is necessary to demonstrate effectively the intravaginal administration of selected probiotic lactobacilli on the recolonization of the urogenital microbiota, the prevention or therapy of different clinical syndromes, their effect on pregnant women, etc, that will allow the proposal of the bacteriotherapy with lactobacilli as a valid alternative to the other drugs therapies.

Key words
Lactobacilli, prevention, urinary tract infections, probiotic


Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Infectología, Urología
Relacionadas: Bioquímica, Farmacología, Medicina Farmacéutica, Medicina Interna, Obstetricia y Ginecología



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Patrocinio y reconocimiento:
Reconocimiento: Los resultados presentados en este trabajo han dado lugar a las tesis doctorales de los siguientes profesionales: Dra. Clara Silva de Ruiz, Dra. Virginia Ocaña y Dra. Silvina Juárez Tomas.
Los proyectos de investigación cuyos resultados se vuelcan en el presente trabajo han sido financiados por subsidios de CONICET.
Las cepas probióticas seleccionadas se licenciaron a la empresa italiana Anidral, del grupo Molfin-Ace-Probiotical, para su obtención en gran escala y comercialización mundial.
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