Authors' Reports

Anubrata Ghosal
describes for SIIC the most significant aspects of his article
describe para SIIC los aspectos relevantes de su artículo


PEPTIDE NUCLEIC ACID HOLDS THE PROMISE OF NOVEL ANTIBIOTICS

In this current study, we have developed four antisense peptide-PNA conjugates by targeting two widely conserved bacterial genes, acpP and ftsZ. These molecules can completely inhibit the growth of several P. aeruginosa strains at 1-2 μM concentrations.

The article was published by
El artículo fue publicado por
Nucleic Acid Therapeutics
Volume / Volumen: 22
Number / Número: 5
First and last pages / Páginas inicial-final: 323-334
Year / Año: 2012
Month / Mes: october





Principal institution where the research took place
Institución principal de la investigación
The Panum Institute, University of Copenhagen, Copenhagen, Hovedstaden, Denmark


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Bibliographic references
Referencias bibliográficas

1. Stover C-K., Pham X-Q., Erwin A-L., Mizoguchi S-D., Warrener P.,H.M.-J. and Brinkman F-S., Hufnagle W-O., Kowalik D-J., Lagrou M. Garber R-L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L-L., Coulter S-N., Folger K-R., Kas A., Larbig K., Lim R., Smith K., Spencer D., Wong G-K., Wu Z., Paulsen I-T., Reizer J., Sa,O.M.-V. (2000) Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature, 406, 959–964.
2. Walker,T.S., Walker,T.S., Bais,H.P., Bais,H.P., De,E., De,E., Schweizer,H.P., Schweizer,H.P., Rahme,L.G., Rahme,L.G., et al. (2004) Pseudomonas aeruginosa-Plant Root Interactions. Pathogenicity, Biofilm Formation, and Root Exudation. Society, 134, 320–331.
3. Mahajan-Miklos,S., Tan,M.W., Rahme,L.G. and Ausubel,F.M. (1999) Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell, 96, 47–56.
4. Lister,P.D., Wolter,D.J. and Hanson,N.D. (2009) Antibacterial-resistant Pseudomonas aeruginosa: Clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin. Microbiol. Rev., 22, 582–610.
5. Livermore,D.M. (2002) Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clin. Infect. Dis., 34, 634–640.
6. Porras-Gómez,M., Vega-Baudrit,J. and Núñez-Corrales,S. (2012) Overview of Multidrug-Resistant Pseudomonas aeruginosa and Novel Therapeutic Approaches. J. Biomater. Nanobiotechnol., 03, 519–527.
7. Heather K. Allen, Justin Donato, Helena Huimi Wang, Karen A. Cloud-Hansen,J.D.& J.H. (2010) Call of the wild: antibiotic resistance genes in natural environments. Nat. Rev. Microbiol. Rev., 8, 251–259.
8. Good,L. and Nielsen,P.E. (1998) Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA. Proc. Natl. Acad. Sci. U. S. A., 95, 2073–6.
9. Liam Good & Peter E. Nielsen (1998) Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat. Biotechnol., 16, 355–8.
10. Good L, Awasthi SK, Dryselius R, Larsson O,N.P. (2001) Bactericidal antisense effects of peptide–PNA conjugates. Nat. Biotechnol., 19, 360–364.
11. Jeon B,Z.Q. (2009) Sensitization of Campylobacter jejuni to fluoroquinolone and macrolide antibiotics by antisense inhibition of the CmeABC multidrug efflux transporter. J Antimicrob Chemother, 63, 946–8.
12. Nekhotiaeva N, Awasthi SK, Nielsen PE, and G.L. (2004) Inhibition of Staphylococcus aureus Gene Expression and Growth Using Antisense Peptide Nucleic Acids. Mol. Ther., 10, 652–9.
13. Kulyté A, Nekhotiaeva N, Awasthi SK,G.L. (2005) Inhibition of Mycobacterium smegmatis gene expression and growth using antisense peptide nucleic acids. J Mol Microbiol Biotechnol., 9, 101–9.
14. Parthiban Rajasekaran, Jeffry C. Alexander, Mohamed N. Seleem, Neeta Jain, Nammalwar Sriranganathan, Alice R. Wattam, João C. Setubal,S.M.B. (2013) Peptide nucleic acids inhibit growth of Brucella suis in pure culture and in infected murine macrophages. Int. J. Antimicrob. Agents, 41, 358–362.
15. Ghosal,A. and Nielsen,P.E. (2012) Potent antibacterial antisense peptide-peptide nucleic acid conjugates against Pseudomonas aeruginosa. Nucleic Acid Ther., 22, 323–34.
16. Kurupati P, Tan KS, Kumarasinghe G,P.C. (2007) Inhibition of gene expression and growth by antisense peptide nucleic acids in a multiresistantbeta-lactamase-producing Klebsiella pneumoniae strain. Antimicrob Agents Chemother, 51, 805–11.
17. Ghosal,A., Vitali,A., Stach,J.E.M. and Nielsen,P.E. (2013) Role of SbmA in the uptake of peptide nucleic acid (PNA)-peptide conjugates in E. coli. ACS Chem. Biol., 8, 360–7.
18. Bai H, You Y, Yan H, Meng J, Xue X, Hou Z, Zhou Y, Ma X, Sang G,L.X. (2012) Antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpoD gene. Biomaterials, 33, 659–67.
19. Patenge,N., Pappesch,R., Krawack,F., Walda,C., Mraheil,M.A., Jacob,A., Hain,T. and Kreikemeyer,B. (2013) Inhibition of Growth and Gene Expression by PNA-peptide Conjugates in Streptococcus pyogenes. Mol. Ther. Nucleic Acids, 2, e132.
20. Braasch,D. a, Nulf,C.J. and Corey,D.R. (2002) Synthesis and purification of peptide nucleic acids. Curr. Protoc. Nucleic Acid Chem., Chapter 4, Unit 4.11.
21. Ghosal,A. (2012) Novel antibacterial agents ( antibiotics ) based on RNA interference using Peptide Nucleic Acid ( PNA ). 10.1021/cb300434e.Appendix.

Other articles written by the author Anubrata Ghosal
Otros artículos de Anubrata Ghosal





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Anubrata Ghosal was invited by SIIC on
Anubrata Ghosal fue invitado por SIIC el
2013, january 25
  The authors' report was accepted on
La crónica del autor fue aprobada el
2021, march 9
The authors' report was accepted on
La crónica del autor fue aprobada el
2021, march 9
Published in siicsalud
Publicado en siicsalud
2021, june 7





The article is strictly related to the following sections of siicsalud
El artículo se relaciona estrictamente con las siguientes secciones de siicsalud

 
Genetics
/ Genética Humana
 
Infectious Diseases
/ Infectología
 

and secondarily related to the following sections

y secundariamente con las siguientes secciones

Biochemistry
Bioquímica

Inmunology
Inmunología

Information about the full text
Acerca del trabajo completo


Potent Antibacterial Antisense Peptide-Peptide Nucleic Acid Conjugates Against Pseudomonas Aeuruginosa


Author / Autor
Anubrata Ghosal1

1 Dr., Massachusetts Institute Of Technology (mit), Boston, EE.UU., Postdoctoral Associate


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Nucleic Acid Therapeutics

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