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Crónicas de autores

Carolina Andrea Pontillo *

Autora invitada por SIIC

Este trabajo representa una contribución significativa a la comprensión del mecanismo molecular de acción de un pesticida organoclorado como el Hexaclorobenceno en la carcinogénesis mamaria

ACTIVACIÓN DE LAS VÍAS DE SEÑALIZACIÓN DE C-SRC/HER1/STAT5B Y HER1/ERK1/2 Y DE LA MIGRACIÓN CELULAR POR HEXACLOROBENCENO EN LA LÍNEA CELULAR DE CARCINOMA MAMARIO HUMANO MDA-MB-231

Demostramos que el pesticida organoclorado Hexaclorobenceno estimula la migración celular y las vías de señalización c-Src/HER1/STAT5b y HER1/ERK1/2 en la línea de carcinoma mamario humano MDA-MB-231. Observamos que c-Src, HER1 y el Receptor de Hidrocarburos Aromáticos (AhR) están involucrados en la acción del pesticida sobre la migración celular. Este trabajo representa una contribución significativa a la comprensión del mecanismo molecular de acción del HCB en la carcinogénesis mamaria.

*Carolina Andrea Pontillo
describe para SIIC los aspectos relevantes de su trabajo
ACTIVATION OF C-SRC/HER1/STAT5B AND HER1/ERK1/2 SIGNALING PATHWAYS AND CELL MIGRATION BY HEXACHLOROBENZENE IN MDA-MB-231 HUMAN BREAST CANCER CELL LINE
Toxicological Sciences,
120(2):284-296 Abr, 2011

Esta revista, clasificada por SIIC Data Bases, integra el acervo bibliográfico
de la Biblioteca Biomédica (BB) SIIC.

Institución principal de la investigación
*Universidad de Buenos Aires, Buenos Aires, Argentina
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Referencias bibliográficas
Alvarez, L. , Randi, A., Alvarez, P., Piroli, G., Chamson-Reig, A., Lux-Lantos, V., Kleiman de Pisarev, D. (2000). Reproductive effects of hexachlorobenzene in female rats. J. Appl. Toxicol. 20, 81-7. ATSDR. (2002). Toxicological profile for hexachlorobenzene. U.S.D.O.H.A.H., Services. Barouki, R., Coumoul, X., Fernandez-Salguero, P. M. (2007). The aryl hydrocarbon receptor, more than a xenobiotic-interacting protein. FEBS Lett. 581, 3608-3615. Bartucci, M., Morelli, C., Mauro, L., Andó, S., Surmacz, E. (2001). Differential insulin-like growth factor I receptor signaling and function in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells. Cancer Res. 61, 6747-54. Bernaciak, T. M., Zareno, J., Parsons, J. T. y Silva, C. M. (2009). A novel role for signal transducer and activator of transcription 5b (STAT5b) in β1-integrin-mediated human breast cancer cell migration. Breast Cancer Res. 11, 1-11. Biscardi J. S., Belsches, A. P., Parsons, S. J. (1998). Characterization of human epidermal growth factor receptor and c-Src interactions in human breast tumor cells. Mol. Carcinog. 21, 261-272. Biscardi, J. S., Maa, M. C., Tice, D. A., Cox, M. E., Leu, T. H., Parsons, S. J. (1999). c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function. J. Bio. Chem. 274, 8335–43. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72, 248-54. Bulayeva, N. N. and Watson, C. S. (2004). Xenoestrogen-induced ERK1 and ERK-2 activation via multiple membrane-initiated signaling pathways. Environ. Health Perspectives 112, 1481-1487. Chen, J. X, Zeng, H., Tuo, Q. H., Yu, H., Meyrick, B. and Aschner, J. L. (2007). NADPH oxidase modulates myocardial Akt, ERK1/2 activation, and angiogenesis after hypoxia-reoxygenation. Am. J. Physiol. Heart Circ. Physiol. 292, 1664–1674. Dimri, M., Naramura, M., Duan, L., Chen, J., Ortega-Cava, C., Chen, G., Goswami, R., Fernandes, N., Gao, Q., Dimri, G. P., Band, V., Band, H. (2007). Modeling breast cancer-associated c-Src and EGFR overexpression in human MECs: c-Src and EGFR cooperatively promote aberrant three-dimensional acinar structure and invasive behavior. Cancer Res. 67, 4164-72. Diry M, Tomkiewicz C, Koehle C, Coumoul X, Bock KW, Barouki R, Transy C. (2006). Activation of the dioxin/aryl hydrocarbon receptor (AhR) modulates cell plasticity through a JNK-dependent mechanism. Oncogene 25, 5570-5574. Ezendam, J., Vissers, I., Bleumink, R., Vos, J.G., Pieters, R. Immunomodulatory effects of tetrachlorobenzoquinone, a reactive metabolite of hexachlorobenzene. (2003). Chem. Res. Toxicol. 16, 688-94. Frame, M. C. (2002). Src in cancer: deregulation and consequences for cell behaviour. Biochim. Biophys. 1602, 114–130. García, M. A., Peña, D., Alvarez, L., Cocca, C., Pontillo, C., Bergoc, R., de Pisarev, D. K., Randi, A. (2010). Hexachlorobenzene induces cell proliferation and IGF-I signaling pathway in an estrogen receptor alpha-dependent manner in MCF-7 breast cancer cell line. Toxicol. Lett. 192, 195-205. Haarmann-Stemmann, T., Hanno, B., Josef, A. (2009). Growth factors, cytokines and their receptors as downstream targets of aryl hydrocarbon receptor (AhR) signaling pathways. Biochemical Pharmacology 77, 508 – 520. Hahn, M. E., Goldstein, J. A., Linko, P., Gasiewicz, T. A. (1989). Interaction of Hexachlorobenzene with the receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro and in vivo. Arch. Biochem. Biophys. 270, 344-355. Han, E. H., Kim, H.G., Hwang, Y. P., Choi, J. H., Im, J. H., Park, B., Yang, J. H., Jeong, T. C., Jeong, H. G. (2010). The role of cyclooxygenase-2-dependent signaling via cyclic AMP response element activation on aromatase up-regulation by o,p'-DDT in human breast cancer cells. Toxicol. Lett. [Epub ahead of print]. Kleiman de Pisarev, D. L, Rios de Molina, M.C., San Martin de Viale, L. C. (1990). Thyroid function and thyroxine metabolism in hexachlorobenzene-induced porphyria. Biochem Pharmacol.1 39, 817-25. Kloth, M. T., Laughlin, K. K., Biscardi, J. S., Boerner, J. L., Parsons, S.J., Silva, C. M. (2003) STAT5b, a Mediator of Synergism between c-Src and the Epidermal Growth Factor Receptor. JBC 278, 1671-79. Kohle, C., Gschaidmeier, H., Lauth, D., Topell, S., Zitzer, H., Bock, K. W. (1999) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)- mediated membrane translocation of c-Src protein kinase in liver WB-F344 cells. Arch. Toxicol. 73, 152–158. Koppikar, P., Choi, S. H., Egloff, A. M., Cai, Q., Suzuki, S., Freilino, M., Nozawa, H., Thomas, S. M., Gooding, W. E., Siegfried, J. M., Grandis, J. R. (2008). Constitutive activation of signal transducer and activator of transcription 5 contributes to tumor growth, epithelial-mesenchymal transition, and resistance to epidermal growth factor receptor targeting. Clin. Cancer Res. 23, 7682-90. Leaman, D. W., Leung, S., Li, X., Stark, G. R. (1996). Regulation of STAT-dependent pathways by growth factors and cytokines. FASEB J. 10, 1578–1588. Lin, P. H., Lin, C. H., Huang, C. C., Chuang, M. C., Lin, P. (2007) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces oxidative stress, DNA strand breaks, and poly(ADP-ribose) polymerase-1 activation in human breast carcinoma cell lines. Toxicol. Lett. 172, 146-158. Mahon MJ and Gasiewicz AT (1992). Chelatable metal ions are not required for aryl hydrocarbon receptor transformation to a DNA binding form: Phenanthrolines are possible competitive antagonists of 2,3,7,8-tetrachlorodibenzo-p-dioxin. Archives of Biochemistry and Biophysics. 297, 1-8. Matsumura (1994). How important is the protein phosphorylation pathway in the toxic expression of dioxin-type chemicals?. Biochem. Pharmacol. 48, 215-24. Merchant, M., Arellano, L., and Safe, S. (1990). The mechanism of action of alpha-naphthoflavone as an inhibitor of 2,3,7,8-tetrachlorodibenzo-p-dioxininduced CYP1A1 gene expression. Arch. Biochem. Biophys. 281, 84–89. Miller, M. E., Holloway, A. C., Foster, W.G. (2005). Benzo-[a]-pyrene increases invasion in MDA-MB-231 breast cancer cells via increased COX-II expression and prostaglandin E2 (PGE2) output. Clin. Exp. Metastasis 22, 149-156. Mylchreest, E., Charbonneau, M. (1997). Studies on the mechanism of uroporphyrinogen decarboxylase inhibition in hexachlorobenzene-induced porphyria in the female rat. Toxicol. Appl. Pharmacol. 145, 23-33. Ou, Y. C., Conolly, R. B., Thomas, R. S., Xu, Y., Andersen, M. E., Chubb, L.S., Pitot, H. C., Yang, R. S. (2001). A clonal growth model: time-course simulations of liver foci growth following penta- or hexachlorobenzene treatment in a medium-term bioassay. Cancer Res. 61, 1879-89. Park, S., Mazina, O., Kitagawa, A., Wong, P., and Matsumura, F. (2004). TCDD causes suppression of growth and differentiation of MCF10A, human
mammary epithelial cells by interfering with their insulin receptor signaling through c-Src kinase and ERK activation. J. Biochem. Mol. Toxicol. 18,322–331. Parsons, S. J., Parsons, J. T. (2004). Src family kinases, key regulators of signal transduction. Oncogen 23, 7906-9. Plante, I., Charbonneau, M., and Cyr, D. G. (2002). Decreased gap junctional intercellular communication in hexachlorobenzene-induced gender-specific hepatic tumor formation in the rat. Carcinogenesis 23, 1243–1249. Plante, I., Cyr, D. G., and Charbonneau, M. (2005). Involvement of the integrin-
linked kinase pathway in hexachlorobenzene-induced gender-specific rat hepatocarcinogenesis. Toxicol. Sci. 88, 346–357. Qin, H., Shao, Q., Curtis, H., Galipeau, J., Belliveau, D. J., Wang, T., Alaoui-Jamali, M. A., and Laird, D. W. (2002). Retroviral delivery of connexin genes to human breast tumor cells inhibits in vivo tumor growth by a mechanism that is independent of significant gap junctional intercellular communication.J. Biol. Chem. 277, 29132–29138. Randi, A. S., Cocca, C., Carbone, V., Nuñez, M., Croci, M., Gutierrez, A., Bergoc, R., Kleiman de Pisarev, D. L, (2006). Hexachlorobenzene is a tumor Co-carcinogen and induces alterations in Insulin Growth Factors Signaling Pathway in the Rat Mammary Gland. Toxicological Sciences 89, 83-92. Randi, A. S., Sanchez, M. S., Alvarez, L., Cardozo, J., Pontillo, C., Kleiman de Pisarev, D. (2008). Hexachlorobenzene triggers AhR translocation to the nucleus, c-Src activation and EGFR transactivation in rat liver. Toxicol. Lett. 177, 116-22. Rho, O., Kim D. J., Kiguchi, K., DiGiovanni, J. (2010). Growth factor signaling pathways as targets for prevention of epithelial carcinogenesis. Molecular carcinogenesis. [Epub ahead of print]. Tice, D. A., Biscardi, J. S., Nickles, A. L., Parsons, S. J. (1999) Mechanism of biological synergy between cellular Src and epidermal growth factor receptor. Proc. Natl. Acad. Sci. U.S.A. 96, 1415-20. To-Figueras, J., Sala, M., Otero, R., Barrot, C., Santiago-Silva, M., Rodamilans, M., Herrero, C., Grimalt, J., Sunyer, J. (1997). Metabolism of Hexachlorobenzene in humans: Association between serum levels and urinary metabolites in a highly exponed population. Envirom. Health Perspect. 105, 78-83. Toker, A., Yoeli-Lerner, M. (2006) Akt signaling and cancer: surviving but not moving on. Cancer Res. 66, 3963-6.
Verner, M. A., Charbonneau, M., López-Carrillo, L., Haddad, S. (2008). Physiologically based pharmacokinetic modeling of persistent organic pollutants for lifetime exposure assessment: a new tool in breast cancer epidemiologic studies. Environ Health Perspect. 116, 886-92. Wang, C., Navab, R., Iakovlev, V., Leng, Y., Zhang, J., Tsao, M. S., Siminovitch, K., McCready, D. R. , Done, S. J. (2007). Abelson interactor protein-1 positively regulates breast cancer cell proliferation, migration, and invasion. Mol. Cancer Res. 5, 1031-9. Yoeli-Lerner M, Gary K. Yiu, Isaac Rabinovitz, Peter Erhardt, Sebastien Jauliac, and Alex Toker (2005). Akt Blocks Breast Cancer Cell Motility and Invasion through the Transcription Factor NFAT. Molecular Cell 20, 539–550.
Otros artículos de Carolina Andrea Pontillo

1)”Hexachlorobenzene induces cell proliferation and IGF-I signaling pathway in an estrogen receptor α-dependent manner in MCF-7 breast cancer cell line.”. María Alejandra García, Delfina Peña, Laura Alvarez, Claudia Cocca, Carolina Pontillo, Rosa Bergoc, Diana Kleiman de Pisarev y Andrea Randi. Toxicology Letters 192: 195-205. (2010).

2)“Hexachlorobenzene triggers apoptosis in rat thyroid follicular cells”. Florencia Chiappini, Laura Alvarez, Victoria Lux-Lantos, Andrea S. Randi, Diana L. Kleiman de Pisarev. Toxicological Sciences 108(2):301-310. (2009).

3)“Hexachlorobenzene triggers AhR translocation to the nucleus, c-Src activation and EGFR transactivation in rat liver”. Randi Andrea, Marcela Susana Sánchez, Laura Alvarez, Julián Cardozo, Carolina Pontillo, Diana Kleiman de Pisarev. Toxicology Letters 177: 116-122 (2008).

4)“Hexachlorobenzene is a tumor Co-carcinogen and induces alterations in Insulin Growth Factors Signaling Pathway in the Rat Mammary Gland”. Randi, Andrea S, Cocca C, Carbone V, Nuñez M, Croci M, Gutierrez A, Bergoc R, Kleiman de Pisarev DL.
Toxicological Sciences 89 (1): 83-92 (2006).

5) “Hexachlorobenzene-induced early changes in Ornithine decarboxylase and Protein Tyrosine Kinase activities, Polyamines and c-Myc, c-Fos and c-Jun proto-oncogenes in rat liver”. Randi, Andrea S.; Hernández, S.; Alvarez, L.; Sánchez, M.; Schwarcz, M.; and D.L. Kleiman de Pisarev. Toxicological Sciences 76: 291-298 (2003).
6) “Effect of in vivo administered hexachlorobenzene on epidermal growth factor receptor levels, protein tyrosine kinase activity and phosphotyrosine content in rat liver”. Andrea S.Randi, H.A.Sancovich, A.M.Ferramola de Sancovich, A.Loaiza, R.Kölliker Frers,, F.Spinelli, and D.L.Kleiman de Pisarev. Biochemical Pharmacology vol 65 (9) 1495-1506 (2003).
7) “Reproductive effects of Hexachlorobenzene in female rats”. L.Alvarez, Andrea Randi, P.Alvarez, G.Piroli, A.Chamson-Reig, V.Lux-Lantos, and Kleiman de Pisarev, DL. J. of Applied Toxicology, 20, 81-87 (2000).

8)- “Effect of Hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue”. L.Alvarez, Andrea Randi, P.Alvarez, R.Kolliker Frers, and D.L.Kleiman de Pisarev. J. of Endocrinological Investigation, 22, 436-445 (1999).
9)-“Hexachlorobenzene, a dioxin type compound, increase Malic Enzyme gene transcription through a mechanism involving the thyroid hormone response element”. A.Loaiza Pérez, M.T.Seisdedos, D.L. Kleiman de Pisarev, H.A.Sancovich, Andrea S.Randi, A.M.Ferramola de Sancovich and P.Santisteban. Endocrinology, 140, 4142- 4151 (1999).

10) “Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function”. Andrea S.Randi, H.A.Sancovich, A.M.Ferramola de Sancovich, A.Loaiza, L.Krawiec and D.Kleiman de Pisarev. Toxicology 125, 83-94 (1998).ISSN: 0300-483X

Para comunicarse con Carolina Andrea Pontillo mencionar a SIIC como referencia:
andybiol@yahoo.com.ar

Autora invitada
24 de mayo, 2011
Descripción aprobada
16 de junio, 2011
Reedición siicsalud
13 de junio, 2012

Acerca del trabajo completo
ACTIVACIÓN DE LAS VÍAS DE SEÑALIZACIÓN DE C-SRC/HER1/STAT5B Y HER1/ERK1/2 Y DE LA MIGRACIÓN CELULAR POR HEXACLOROBENCENO EN LA LÍNEA CELULAR DE CARCINOMA MAMARIO HUMANO MDA-MB-231

Título original en castellano
ACTIVACION DE LAS VIAS DE SEÑALIZACION DE C-SRC/HER1/STAT5B Y HER1/ERK1/2 Y DE LA MIGRACION CELULAR POR HEXACLOROBENCENO EN LA LINEA CELULAR DE CARCINOMA MAMARIO HUMANO MDA-MB-231

Autor


Acceso a la fuente original
Toxicological Sciences
http://www.toxsci.oupjournals.org

El artículo se relaciona estrictamente con las especialidades de siicsalud


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